Alternative Oxidase of Potato Is an Integral Membrane Protein Synthesized de Novo during Aging of Tuber Slices
نویسندگان
چکیده
منابع مشابه
Activation of protein synthesis in aging potato tuber slices.
LiCI-extraction of RNAs from isolated ribosomes, however, destroys the coding properties of mRNAs (as has been shown with cerebral mRNA; P. MANDEL, pers. comm.). Therefore separation of mRNAs from ribosomes was achieved by EDTA. If isolated ribosomes from intact potato tubers are dissociated into subunits and RNAs by EDTA, sucrose density gradient profiles show the presence of the 60Sand 40S-su...
متن کاملChanges in sucrose synthetase activities in aging potato tuber slices.
The me-chanism by which the reversible conversion of starch ito sucrose takes place in potato tubers exposed t,o cold is as yet essentilally unknown. Early 'studies on the subjectt tried to correllate this conversion with va,riations in the activity of related enzymes (10). Those responsible for the synthesis of sucrose and sticrose-6--phosphiate (1) are undoubtedly highly relevant. In add'itio...
متن کاملAlteration of Coding Properties of Polysome-Associated Messenger RNA in Potato Tuber Slices during Aging.
Kinetics of polysome formation, translational capacity, and coding properties of polysome-associated messenger RNA were investigated in potato tuber tissue discs during aging. Polysome content rapidly increased immediately after slicing from 14% of total ribosomes in freshly sliced discs to 55% within 12 hours of aging. The amount of polysomal RNA also increased 5-fold during this period. Trans...
متن کاملStructure, gas chromatographic measurement, and function of suberin synthesized by potato tuber tissue slices.
The polymeric material (suberin) of the wound periderm of potato tuber slices was analyzed after depolymerization with LiAIH(4) in tetrahydrofuran or BF(3) in methanol with the use of thin layer chromatography, chemical modification, and combined gas-liquid chromatography and mass spectrometry. Fatty acids (C(16) to C(26)), fatty alcohols (C(16) to C(26)), octadec-9-ene-1, 18-dioic acid, and 18...
متن کاملCitric-acid Induced Loss of Rigidity in Potato Tuber Slices.
and petiole, the interveinal tissue did not reach tritium equality with major veins, the stem, or the external solution. Water of the interveinal tissue maintained a stable tritium content at 60 to 70 % of external concentration for long periods. The pattern of results obtained was similar in sunflower and tobacco. Tritium content of leaf-tissue water vapor transpired by the canopy of an intact...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1990
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.93.1.312